Sunday, May 31, 2015

Laminar Flows, Differences and How to Chose?

Let's start this blog with something that I think is often not clear: the differences between laminar flow cabinets and biological safety cabinets.

I hope this summary table below helps start to unfold these differences, in protection, in usages, etc.
This is obviously the overview of an issue that entails many other discussions and will be brought up recurrently herein.


Type
Protects
Does Not Protect
Usage Examples
Do not use if
Other versions and variants
Laminar Flow Cabinets
Sample
User
Environment
Media Pouring
Sample may contain pathogens or harmful substances
Horizontal and Vertical laminar flows
Biological Safety Cabinets
Class I
User
Environment
Sample
Microbiology
Sample cannot suffer contamination
Ducted or non-ducted,
(carbon filters)
Class II
Sample
User
Environment
—*
Tissue Culture
Sample contains certain radioactive isotopes
Ducted or non-ducted,
30% exhaust and 70%
recirculation in the UK/EU,
double HEPA exhaust in the UK,
(carbon filters)

* To achieve protection of both the sample and the user, these cabinets make use of a very delicate balance between air flows. These are very prone to disruption by environmental conditions and manipulation practices. Analysis of room conditions, cabinet positioning, KI testing, user training and best practices are all crucial to obtain the level of containment required.